Table 1.
Human endoneurial endothelial cell adherens and tight junction protein expression.
| PROTEIN | CELL LINE | mRNA | Protein (WB) | ICC | Cellular Localization |
|---|---|---|---|---|---|
| β-Catenin | pHEndEC (26) | + | + | N/A | N/A |
| VE-Cadherin | pHEndEC (26) | + | + | + | Intercellular Membrane |
| ZO-1 | pHEndEC (14, 26) | + | + | + | Intercellular Membrane |
| FH-BNB (19) | + | N/A | + | Intercellular Membrane | |
| ZO-2 | FH-BNB (19) | + | N/A | + | Intercellular Membrane |
| Occludin | pHEndEC (14, 26) | + | + | + | Intercellular Membrane |
| PnMEC (17, 18) | + | + | N/A | N/A | |
| FH-BNB (19) | + | N/A | + | Intercellular Membrane | |
| Claudin-5 | pHEndEC (14, 26) | + | + | + | Intercellular Membrane |
| PnMEC (16, 17, 18) | + | + | N/A | N/A | |
| FH-BNB (19) | + | + | + | Intercellular Membrane | |
| Claudin-1 | pHEndEC (14) | + | N/A | + | Nuclear |
| FH-BNB (19) | + | N/A | N/A | N/A | |
| Claudin-2 | pHEndEC (14) | + | N/A | + | Perinuclear |
| Claudin-12 | FH-BNB (19) | + | N/A | N/A | N/A |
| Claudin-19 | FH-BNB (19) | + | N/A | N/A | N/A |
| JAM-A | pHEndEC (14, 26) | + | + | + | Cytoplasmic |
| FH-BNB (19) | + | N/A | N/A | N/A | |
Key: pHEndEC: primary human endoneurial endothelial cells, PnMEC: peripheral nerve microvascular endothelial cells, FH-BNB: conditionally immortalized human peripheral nerve microvascular endothelial cells, mRNA: messenger RNA, WB: western blot, ICC: immunocytochemistry, +: detected, N/A: not assessed. Parentheses represent reference numbers.