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. Author manuscript; available in PMC: 2015 May 19.
Published in final edited form as: Curr Biol. 2014 May 1;24(10):1138–1144. doi: 10.1016/j.cub.2014.03.053

Figure 1. Simultaneous visualization of Hsp70 transgenes, nascent transcripts, and nuclear speckles.

Figure 1

(A–B) Hsp70 plasmid pSP14-14-5′UTR-MS2 (A) and the modified SON BAC (B) transgenes. (C–F) DAPI-stained nuclei (blue) versus Hsp70 transgenes (GFP-LacI, bright grey-scale), speckles (GFP-SON, less bright grey-scale) and Hsp70 transcripts (MS2-binding protein-mCherry, red): After heat shock (HS), nascent transcripts (arrowhead) accumulate near speckle (C). Peripheral transgenes (arrowheads) prior to HS (D). Hsp70 transgenes (arrowheads) not associated (E) or associated (F) with speckles. (G) Speckle association index before or after 30 min HS. (H) Disrupting nuclear actin polymerization abolishes HS-induced speckle association. SEM error bars, scale bar = 2μm.