FIG. 2.

Characterization of porcine iPSCs. (A–D′) iPSC lines derived from all PEF lines were dome shaped and similar in morphology to mouse ESCs colonies (A–D, L1, L6, C4, C5), and had a normal karyotype of 38 chromosomes (A′–D′). Scale bar, 100 μm. (E–E′) Proliferation characteristics of the PEF and iPSC lines. (F) RT-PCR assessment showed that both the exogenous (Oct4-V, Myc-V) and the endogenous pluripotency genes (Oct4-en, Klf-en) were expressed in iPSCs. The number of each type cells for RT-PCR was ∼10³ and the number of cycles was 30.