Table 1.
Primer Sequences and PCR Conditions Used for qRT-PCR
| Gene | Primer sequences (5′-3′) | Accession no. | Tm | Product size (bp) |
|---|---|---|---|---|
| Oct-4 | F: GTGGAGGGATGGCCTACTGT | NM_174580 | 59 | 259 |
| R: TTCTGCTTTAGGAGCTTGGCA | ||||
| Sox-2 | F: TTCTTCGCCTGATTTTCCTC | NM_001105463 | 60 | 275 |
| R: GGGCTGTTCTTCTGGTTGCC | ||||
| c-Myc | F: TACAACATCCGAGCGACACC | NM_001046074 | 58 | 229 |
| R: TGCACCGAATCGTAGTCGAG | ||||
| Klf-4 | F: GGTCCCACCGCTCCATTAC | NM_001105385 | 60 | 247 |
| R: ATCTGAGCGGGCAAACTTCC | ||||
| Col1a1 | F: CCAGCCGCAAAGAGTCTACA | NM_00103403 | 59 | 296 |
| R: GGGACTTTGGCGTTAGGACA | ||||
| Col1a2 | F: CCACTGGAGAAATCGGACCC | NM_174520 | 61 | 280 |
| R: CTGCCGTCAATACCAGGGAG | ||||
| Gapdh | F: CCTGCCCGTTCGACAGATAG | NM_001034034 | 59 | 249 |
| R: CCGTTCTCTGCCTTGACTGT |
F, forward primer; R, reverse primer; Tm, annealing temperature.