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. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: Cancer Res. 2014 Mar 19;74(10):2785–2795. doi: 10.1158/0008-5472.CAN-13-3176

Figure 6. CLPTM1L overexpression increases the fraction of aneuploid cells.

Figure 6

(A) PANC-1 cells stably expressing WT N-FLAG CLPTM1L (right) and HEK293T cells transiently expressing N-FLAG CLPTM1L (left) and were labeled with BrDU and analyzed for cell cycle content. The percentage of aneuploid cells (DNA content >G2M peak) is shown in the graphs. (B) DNA content was also measured in PANC-1 cells stably expressing WT N-FLAG CLPTM1L or mutant N-FLAG CLPTM1L (CLPTM1L-ΔCterm or CLPTM1L-ΔLoop) with transient siRNA knock-down of non-muscle myosin II MYH9 or a non-target (NEG) siRNA. The percentage of aneuploid cells was compared between cells expressing WT and mutant CLPTM1L and cells containing empty vector. The average and SE of n=3 replicates is shown in the graphs.