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. Author manuscript; available in PMC: 2014 May 22.
Published in final edited form as: Chem Res Toxicol. 2009 Jun;22(6):1189–1193. doi: 10.1021/tx900107q

Figure 3.

Figure 3

(A) Calculated ECD of S and R-allantoin enantiomers (B) Experimental ECD spectra of S-allantoin (blue) and R-allantoin (red). The experimental ECD spectra were adapted from Figure 2A of Ramazzina et al (34), with the gracious permission of Prof. Riccardo Percudani. These ECD data were obtained using (S)-allantoin (0.2 mM) or (R,S)-allantoin (0.4 mM) as substrates in the presence of PuuE (0.37 μM) in1ml of 0.1 M potassium phosphate, pH 7.6.