Fig. 4.

(A) TIRF imaging revealed a cluster-like attachment of drebrin (green) to actin filaments (red), providing the first indications of cooperative interaction between drebrin_1–300 and F-actin. (B) AFM images of F-actin decorated with drebrinA_1–300 at sub-saturating ratios reveal their cooperative binding (binding clusters are marked by yellow arrows along individual filaments). (C) and (D) show drebrin clusters along the actin filament at higher resolution. (E) Identification of the segments of drebrin free F-actin; 1^st, 2^nd and 3^rd neighbor relative to the drebrin-bound helix in the AFM image of partially decorated Drb_1–300–F-actin. Adapted from Sharma S. et al.⁸⁴ with permission from Cell Press.