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More Hsp104 binds to weak prion fibers than to strong prion fibers in vivo. The fluorescence recovery after photobleaching curves for Hsp104-mCherry containing foci in strong (blue) and weak (brown) [PSI+] are shown. The traces were normalized for both the initial intensity and the depth of photobleaching. Figure S3 shows that Hsp104-mCherry does not impact chaperone function.
