Figure 4.

Effect of ADSL overexpression on hepatic glucose output and insulin sensitivity. Rat primary hepatocytes were cultured under normal Normal or metabolic overload (MO) conditions. At the end of 24 hours the hepatic glucose output was estimated by measuring the glucose released into the culture media either in the absence (control) or presence of 10nM of insulin (+insulin) as explained in materials and methods. Glucose released into the media was normalized to total cellular protein. Unpaired students t-test was used to calculate the statistical significance ** (p < 0.01).