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Effect of ADSL overexpression on inflammatory cytokines. Rat primary hepatocytes were cultured under Normal or metabolic overload (MO) conditions for 24 hours. The m-RNA levels of MCP-1 (A) or IL-1b (B) were checked by real time quantitative PCR. Beta actin was used as endogenous control. Unpaired students t-test was used to calculate the statistical significance * (p < 0.05), ***(p < 0.001).
