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. 2014 Apr 24;13:51. doi: 10.1186/2251-6581-13-51

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Copyright © 2014 Sadasivan et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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ADSL overexpression in HFD mouse liver. Mice were fed on high fat diet for 13 weeks and were divided into two groups (n = 8 in each group). For one group, DNA construct expressing the ADSL gene was injected through tail vein (HFD + ADSL), and control group received plasmid that does not express ADSL (HFD control). The gene delivery was conducted every. At the end of the study, (4 weeks later) RNA was isolated from liver and ADSL gene expression levels were quantified using Q-PCR. b-actin was used as house keeping gene control. Unpaired students t-test was used to calculate the statistical significance * (p < 0.05), ***(p < 0.001).