Table 1.
Transgenic lines for optogenetic manipulation of mammalian nervous system function
| Line name | Species | Strategy | Opsin variant | Reporter | Cellular promoter | Reported opsin expression/induction | Line availability | Citation |
|---|---|---|---|---|---|---|---|---|
| Thy1.2-ChR2-EYFP line 9 | mouse | Plasmid transgenic | ChR2 | EYFP fusion | Thy1.2 | Layer V cortical pyramidal neurons,
hippocampal CA1 and CA3 pyramidal neurons, various nuclei of the thalamus, midbrain and brainstem, retinal ganglion cells |
JAX stock 007615 | [42.43] |
| Thy1.2-ChR2-EYFP line 18 | mouse | Plasmid transgenic | ChR2 | EYFP fusion | Thy1.2 | Layer V cortical pyramidal neurons,
hippocampal CA1 and CA3 pyramidal neurons, various nuclei of the thalamus, midbrain and brainstem, cerebellar mossy fibers, retinal ganglion cells |
JAX stock 007612 | [42.43] |
| Thy1.2-ChR2(H134R)-EYFP line 20 | mouse | Plasmid transgenic | ChR2(H134R) | EYFP fusion | Thy1.2 | Cortex, CA1, thalamus, superior colliculus, inferior colliculus, brainstem, amygdala and cerebellum | JAX stock 012350 | unpublished |
| WTChR2V4 | Wistar rat | Plasmid transgenic | ChR2 | Venus fusion | Thy1.2 | Retinal ganglion cell layer and inner
plexiform layer of the retina, mechanoreceptive dorsal root ganglion
neurons, hippocampal neurons. |
contact investigator | [57,58,59] |
| Thy1.2-VChR1-EYFP line 1 | mouse | Plasmid transgenic | VChR1 | EYFP fusion | Thy1.2 | Layer V cortical pyramidal neurons,
hippocampus, thalamus, superior colliculus, inferior
colliculus, brainstem, amygdala, and cerebellum. (Note: sparse labeling) |
JAX stock 012341 | unpublished |
| Thy1.2-VChR1-EYFP line 8 | mouse | Plasmid transgenic | VChR1 | EYFP fusion | Thy1.2 | Cortical layer II/III and V pyramidal neurons,
hippocampus, thalamus, superior colliculus, inferior
colliculus, brainstem, amygdala, and cerebellum. (Note: widespread labeling) |
JAX stock 012348 | unpublished |
| Omp-ChR2-EYFP (ORC-M) | mouse | Plasmid transgenic | ChR2 | EYFP fusion | Omp | Mature olfactory sensory neurons of the olfactory epithelium, vomeronasal organ and accessory olfactory bulb | contact investigator | [60] |
| Thy1.2-NpHR-EYFP line 6 | mouse | Plasmid transgenic | NpHR | EYFP fusion | Thy1.2 | Layer V cortical pyramidal neurons,
hippocampal CA1 and CA3 pyramidal neurons, dentate granule
cells, superior and inferior colliculus, thalamus and brain stem. (Note: sparse labeling and intracellular aggregation) |
no longer available | [88] |
| Thy1.2-eNpHR-EYFP line 2 | mouse | Plasmid transgenic | eNpHR2.0 | EYFP fusion | Thy1.2 | Layer V cortical pyramidal neurons,
hippocampal CA1 and CA3 pyramidal neurons, dentate granule
cells, subiculum, thalamus, superior and inferior colliculus, brainstem, amygdala, and cerebellum |
JAX stock 012332 | unpublished |
| Thy1.2-eNpHR-EYFP line 4 | mouse | Plasmid transgenic | eNpHR2.0 | EYFP fusion | Thy1.2 | Layer II/III and V cortical pyramidal neurons,
hippocampus (interneuron), subiculum, thalamus, superior and inferior colliculus, brainstem, amygdala, globus pallidus (interneuron), and cerebellum |
JAX stock 012334 | unpublished |
| Vglut2-ChR2(H134)-EYFP | mouse | BAC transgenic | ChR2(H134R) | EYFP fusion | Vglut2 | Glutamatergic neurons in the hindbrain and brainstem | JAX stock 017978 | [61] |
| VGAT-ChR2(H134R)-EYFP line 8 | mouse | BAC transgenic | ChR2(H134R) | EYFP fusion | VGAT | GABAergic neurons throughout the brain
(cortex, hippocampus, olfactory bulb, thalamic reticular
nucleus, superior and inferior colliculus, the molecular layer of the cerebellum, brain stem) |
JAX stock 014548 | [62] |
| TPH2-ChR2(H134R)-EYFP line 5 | mouse | BAC transgenic | ChR2(H134R) | EYFP fusion | TPH2 | Serotonergic neurons in the dorsal raphe nucleus, median raphe nucleus, and interpeduncular nucleus | JAX stock 014555 | [62] |
| ChAT-ChR2(H134R)-EYFP line 5 | mouse | BAC transgenic | ChR2(H134R) | EYFP fusion | ChAT | Cholinergic neurons in striatum (weak expression) | JAX stocl 014545 | [62] |
| ChAT-ChR2(H134R)-EYFP line 6 | mouse | BAC transgenic | ChR2(H134R) | EYFP fusion | ChAT | Cholinergic neurons in striatum, basal forebrain, facial nucleus, trochlear nucleus, medial habenula, cortex | JAX stock 014546 | [62,67] |
| Pvalb-ChR2(H134R)-EYFP line 15 | mouse | BAC transgenic | ChR2(H134R) | EYFP fusion | Pvalb | Thalamic reticular nucleus neurons, cerebellum Purkinje cells, basket and stellate molecular layer interneurons | JAX stock 012355 | [62] |
| Mrgprd-ChR2(H134R)-Venus | mouse | Knock-in | ChR2(H134R) | Venus fusion | Mrgprd locus | Non-peptidergic spinal cord sensory neuron subset | contact investigator | [70] |
| Omp-ChR2(H134R)-EYFP | mouse | Knock-in | ChR2(H134R) | EYFP fusion | Omp locus | Mature olfactory sensory neurons of the olfactory epithelium | JAX stock 014173 | [72] |
| R26-ChR2(H134R)-EGFP | mouse | Cre-inducible knock-in | ChR2(H134R) | EGFP fusion | CAG from Rosa26 locus | Cortical interneurons (Gad2-CreERT2) | contact investigator | [73] |
| Ai27 | mouse | Cre-inducible knock-in | ChR2(H134R) | tdTomato fusion | CAG from Rosa26 locus | Cortical and hippocampal pyramidal neurons (Emx1-Cre), cholinergic neurons (ChAT-Cre) | JAX stock 012567 | [75] |
| Ai32 | mouse | Cre-inducible knock-in | ChR2(H134R) | EYFP fusion | CAG from Rosa26 locus | Cortical and hippocampal pyramidal neurons
(Emx1-Cre), cholinergic neurons (ChAT-Cre), hippocampal CA1 basket cells, thalamic reticular nucleus neurons (Pvalb-IRES-Cre) |
JAX stock 012569 | [75] |
| Ai35 | mouse | Cre-inducible knock-in | Arch | EGFP fusion | CAG from Rosa26 locus | Cortical and hippocampal pyramidal neurons (Emx1-Cre), cortical pyramidal neurons (CamKIIa-CreERT2) | JAX stock 012735 | [75] |
| Ai39 | mouse | Cre-inducible knock-in | eNpHR3.0 | EYFP fusion | CAG from Rosa26 locus | Cortical pyramidal neurons (Emx1-Cre), cortical pyramidal neurons (CamKIIa-CreERT2) | JAX stock 014539 | [75] |
| R26-2XChETA | mouse | Cre-inducible knock-in | ChR2(E123T/H134R) | tdTomato (cytosolic) | CAG from Rosa26 locus | Cortical fast-spiking interneurons
(Pvalb-IRES-Cre, PPP1R2-Cre), direct pathway-projecting MSNs, dorsal
and ventral striatum (D1R-Cre), indirect pathway-projecting MSNs, dorsal and ventral striatum (A2AR-Cre), cerebellum Purkinje cells, basket cells, stellate cells (Pvalb-IRES-Cre) |
JAX stock 017455 | [76] |
| BTR6 (bitetO-rhodopsin line 6) | mouse | tTA-inducible plasmid transgenic | ChR2 NpHR | mCherry fusion EGFP fusion | tetO | Dorsal striatum medium spiny neurons (ChR2,
functional) Dorsal striatum medium spiny neurons (NpHR, non-functional) |
contact investigator | [81] |
| TetO-ChR2(C128S)-EYFP | mouse | tTA-inducible knock-in | ChR2(C128S) | EYFP fusion | tetO from beta-actin locus | Cerebellum Purkinje cells (Gad67-tTA),
habenula neurons (Htr5B-tTA), inferior olive neurons
(Htr5B-tTA), microglia (Iba1-tTA), cortical astrocytes and Bergmann glia (Mlc1-tTA), oligodendrocytes (PLP-tTA), hippocampal CA1 pramidal neurons (CaMKII-tTA), striatal MSNs (PDE10A2-tTA) |
contact investigator | [82, 84, 85] |