Table 1.

Statistics of the data collection for structural analysis of H⁺,K⁺-ATPase in different conformations

Conformation	Preparation	Cryo-grids used	Micrographs						Resolution	Phase residual	Ref.
			Tilt angles					Total
			0°	20°	45°	60°	70°
E2AlF	Single carbon	121	–	–	–	–	–	–	–	–	–
			–	–	–	–	–	–
			59	10a	28a	–	–	97
			328	465	420	–	–	1213
E2AlF	Carbon sandwich	182	7	48	92	118	54	319	6.5 Å	31.5°	[15]
			70	120	202	206	119	717
			86	205	311	262	217	1081
			274	584	660	430	349	2297
E2BeF	Carbon sandwich	47	11	20	57	–	–	88	8 Å	33.1°	[24]
			38	24	73			135
			47	64	90	–	–	201
			120	156	286	–	–	562
(SCH)E2BeF	Carbon sandwich	97	6	43	128	118	–	295	7 Å	37.1°	[16]
			14	53	203	210		480
			15	59	278	249	–	601
			70	90	542	403	–	1105
(Rb+)E2AlF	Carbon sandwich	78	3	18	68	57	–	146	8 Å	35.6°	[25]
			9	29	88	116		242
			42	87	208	202	–	539
			80	275	410	402	–	1167
(K+)E2AlF	Carbon sandwich	125	6	34	54	69	–	163	8 Å	36.9°	[25]
			38	73	118	126		355
			80	100	180	204	–	564
			443	588	430	551	–	2012

Table shows numbers of ‘micrographs’ used for the structural determination of the indicated conformation of H⁺,K⁺-ATPase (see Materials and Methods for details). Numbers of micrographs obtained (fourth rows), selected for scanning (third rows), processed by MRC program (second rows) and finally merged into a 3D structure (first rows) are indicated. Because all of the tilted images were affected by the image-shift due to specimen charging in the case of the single carbon preparation of the E2AlF crystals, micrographs with recognizable diffraction spots in the direction parallel to the tilt axis (as shown in Fig. 4d) were selected (a).