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. 2014 Jan 20;23(12):3071–3084. doi: 10.1093/hmg/ddu012

Figure 6.

Figure 6.

DNA demethylation in xenotransplanted hESCs and iPSCs. Human fetal testis section (22 weeks) and testis xenografts of H1 hESCs, OSKM and OSKMV human iPSCs were stained for VASA, 5-methylcytosine (5-MC) and 5-hydroxymethlcytosine (5-hMC) in NUMA+ regions. (A) Cross section of a human fetal testis with positive immunostaining for VASA, 5-MC and 5-hMC. Areas in dotted yellow rectangles are shown in higher magnification on the left of each panel. White arrows indicate germ cells with low to none 5-MC signal relative to 5-hMC. (B–D) Cross sections of mouse testes xenografts after transplantation of undifferentiated H1 hESCs, OSKM and OSKMV cells, respectively, immunostained for NUMA, VASA, 5-MC and 5-hMC. Areas in dotted yellow rectangles are shown in higher magnification on the left of each panel. White arrows indicate NUMA + VASA-positive donor cells with low to none 5-MC signal relative to 5-hMC. Non-germ cell containing regions of the xenografts are shown in far right column for OSKM and OSKMV cells. Scale bar, 50 µm.