Figure 3.

Enlarged entheses and spontaneous fractures in BT^KO mice. Representative images of scapula (A), femur (B) and pelvis (C) of control (Ctrl) and BT^KO mice at 17 weeks of age cleaned by Dermestid beetles. Enlarged entheses of BT^KO mice are indicated by black arrows. In particular, note the enlargement of the third trochanter (arrow outline), the attachment site for the ascending tendon of the gluteus maximus muscle, on the BT^KO femur (B), and the 90^o distortion of the BT^KO pelvis (C). Calluses are marked (asterisks) on the ribs of a BT^KO mouse (D). Alizarin red/alcian blue staining of BT^KO ribs found the presence of blue, cartilaginous cores in some calluses (E), indicating that they were the sites of spontaneous fracturing of the ribs. Scale bars: 2 mm.