Figure 6.

Dynamic histomorphometric differences and cellular abnormalities in BT^KO long bones. (A) In vivo double fluorochrome labeling with calcein (green) and alizarin red shows greatly increased labeled bone surfaces in the trabecular portion of BT^KO femur, compared with a control. (B) TRAP staining for osteoclasts (red) is sharply increased in BT^KO bone, compared with control, as shown in trabecular portions of the femur. Immunohistochemical staining (brown) performed on cortical femur showed higher levels of MEPE (C) and E-11 (D), and lower levels of sclerostin (E) for BT^KO bone than those for controls. Scale bars: 50 µm.