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. 2014 Jan 22;23(12):3180–3188. doi: 10.1093/hmg/ddu028

Figure 1.

Figure 1.

Human muscle regenerates in immunodeficient NOD-Rag1null IL2rγnull mice. (A) Human muscle graft at 130 days posttransplantation into mouse hindlimb. Black sutures indicate ends of graft. (B) Hematoxylin and eosin (H&E) stained cross-section of original donor biceps biopsy. (C) H&E stained cross-section of human graft at 14 days posttransplant. Large myofibers continue to degenerate (arrow) and clusters of small myofibers regenerate (arrowhead and box) within original myofiber basal lamina (insert, anti-human spectrin in red, anti-human collagen IV in green). (D) H&E stained cross-section of human graft at 60 days demonstrating increasing size of grouped myofibers. Asterisk indicates neighboring host mouse muscle. (E) H&E stained cross-section of host hindlimb with fully regenerated human graft at 130 days. Graft within dotted lines. Asterisk indicates mouse muscle. Scale bars: 200 µm. (F) Histograms of CSA of myofibers from xenograft at 130 days (left) and donor muscle (right), n = 1300–1400 fibers each.