Figure 4.

Supra-physiological ΔPDZ nNOS expression does not alter the density of the vasculature in the heart. Arterioles and capillaries in the heart were evaluated by anti-α smooth muscle actin immunostaining and capillaries staining, respectively. (A) Representative photomicrographs of arterioles immunostaining (scale bar: top panel 500 µm; bottom panel 100 µm) and capillaries staining (scale bar: top panel 500 µm; bottom panel 50 µm). (B) Quantification of staining results. N = 4, 3 and 6 for BL10, untreated and treated mdx, respectively, in arterioles' staining; N = 4, 4 and 5 for BL10, untreated and treated mdx, respectively, in capillaries staining.