PrP inhibits both spontaneous and seeded fibrillization
of Aβ1–42.
(○) Spontaneous (no seeds added) reaction for Aβ1–42
alone; (▽) Spontaneous reaction for Aβ1–42 in
the presence of PrP; (●) Reaction for Aβ1–42 upon
seeding with preformed Aβ1–42 fibrils; (▼) Reaction
for Aβ1–42 upon seeding with preformed Aβ1–42
fibrils that were preincubated with PrP (Aβ:PrP molar ratio
of 2:1). The final concentration of monomeric Aβ1–42,
PrP, and Aβ1–42 seeds was 10, 0.5, and 1 μM, respectively.
Fibrils used as seeds were presonicated to increase the number of
ends.