Figure 9.

Single-molecule van’t Hoff analyses. (a) Plot of the temperature dependence of the equilibrium constant of the wild-type human telomerase RNA pseudoknot at three urea concentrations. Identical slopes indicate a urea-independent folding enthalpy, while the constant vertical offset indicates a urea-dependent entropy. (b) Plot of the urea-dependent entropy suggesting a linear dependence of folding entropy on urea concentration. (c) van’t Hoff analysis of the mutant pseudoknot under nondenaturing conditions. All entropy and enthalpy changes can be found in Table 2