Table 2. Thermodynamic Parameters from Single-Molecule van't Hoff Analysis.
| [urea], M | ΔH°, kcal/mol | ΔS°, cal/(mol K) |
|---|---|---|
| (a) WT hTR PK | ||
| 9.1 | –79(2) | –267(4) |
| 8.4 | –78(2) | –262(6) |
| 7.7 | –78(1) | –261(4) |
| 0.0 | –78.2(6)b | –250(6)c |
| (b) DKC hTR PK | ||
| 0.0 | –37(2) | –129(5) |
a
Together they predict folding thermodynamics under non-denaturing conditions.
b
Values from common slope analysis (see text for details).
c
Extrapolated values from the linear dependence of the entropy change on urea concentration (see text for details).