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. 2011 Nov 7;1(1):32–47. doi: 10.3390/biom1010032

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© 2011 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Ursolic acid inhibits cellular protein synthesis. (a and b) A549 cells were incubated with various concentrations of ursolic acid for 5 h and then incubated with [³H]L-leucine (a) or [³H]L-glutamine (b) for 2 h in the presence of ursolic acid. Radioactivity incorporated into the acid-insoluble fractions was measured. Radioactivity (%) is shown as means ± SD (n = 3). * P < 0.05 and ** P < 0.01, compared with control; (c to e) A549 cells were preincubated with or without ursolic acid (50 μM) or cycloheximide (10 μM) for 1 h and then incubated with [³H]L-leucine (c), [³H]uridine (d), or [³H]thymidine (e) for 2 h in the presence or absence of those compounds. Radioactivity incorporated into the acid-insoluble fractions was measured. Radioactivity (%) is shown as means ± SD (n = 3). * P < 0.05 and ** P < 0.01, compared with control; (f) Luciferase mRNA was translated by rabbit reticulocyte lysates in the presence of indicated concentrations of ursolic acid at 30 °C for 90 min. Luciferase activity (%) is shown as means ± SD (n = 3). * P < 0.01, compared with control.

Ursolic acid inhibits cellular protein synthesis. (a and b) A549 cells were incubated with various concentrations of ursolic acid for 5 h and then incubated with [³H]L-leucine (a) or [³H]L-glutamine (b) for 2 h in the presence of ursolic acid. Radioactivity incorporated into the acid-insoluble fractions was measured. Radioactivity (%) is shown as means ± SD (n = 3). * P < 0.05 and ** P < 0.01, compared with control; (c to e) A549 cells were preincubated with or without ursolic acid (50 μM) or cycloheximide (10 μM) for 1 h and then incubated with [³H]L-leucine (c), [³H]uridine (d), or [³H]thymidine (e) for 2 h in the presence or absence of those compounds. Radioactivity incorporated into the acid-insoluble fractions was measured. Radioactivity (%) is shown as means ± SD (n = 3). * P < 0.05 and ** P < 0.01, compared with control; (f) Luciferase mRNA was translated by rabbit reticulocyte lysates in the presence of indicated concentrations of ursolic acid at 30 °C for 90 min. Luciferase activity (%) is shown as means ± SD (n = 3). * P < 0.01, compared with control.