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Ursolic acid inhibits Na+/K+-ATPase activity. Na+/K+-ATPase highly purified from porcine kidney was preincubated with various concentrations of ursolic acid for 15 min and then incubated with ATP for 30 min in the presence of ursolic acid. The inorganic phosphates produced by ATP hydrolysis were measured. Data points represent means ± SD (n = 3). * P < 0.01, compared with control.
