Table 2.

The effect of FTY720 vinylphosphonate on S1P formation from sphingosine in human lung endothelial cells. [³²P]-labeled cells were treated with sphingosine (2 μM) for 30 min. Cells were preteated with either 10 μM (S)-FTY720 vinylphosphonate ((S)-vinylPn) or 10 μM (R)-FTY720 vinylphosphonate ((R)-vinylPn) for (A) 3 h or (B) 24 h. The [³²P] S1P formed is normalized to 100,000 dpm in total lipid extracts. Results are means +/− SD for n = 3 samples.

(A)	[32P] S1P formed (dpm)
Vehicle	878 +/− 6
Sphingosine	4025 +/− 47
(S)-vinylPn + Vehicle	1124 +/− 58
(S)-vinylPn + Sphingosine	5150 +/− 18
(R)-vinylPn + Vehicle	640 +/− 40
(R)-vinylPn + Sphingosine	2326 +/− 32
(B)	[32P] S1P formed (dpm)
Vehicle	1015 +/− 69
Sphingosine	3665 +/− 264
(S)-vinylPn + Vehicle	1011 +/− 35
(S)-vinylPn + Sphingosine	5530 +/− 358
(R)-vinylPn + Vehicle	724 +/− 84
(R)-vinylPn + Sphingosine	2627 +/− 178