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. 2014 Feb 20;4(1):235–251. doi: 10.3390/biom4010235

Table 1.

Overview of refolding procedure (conditions) for the techniques in this review.

Technique Recovery yield Concentration of denatured protein Time Temperature
Dialysis ≤40% [13] 1–10 mg/mL 1–2 days 4 °C
Dilution (chemical additive) ≥80% [14] 1–10 mg/mL 1–2 days 4 °C
Microfluidic chip ≥70% [15] ≥250 μg/mL ≥10 min ≥25 °C