Figure 14.

Effects of treatments on tumor area and osteolysis. Mice were treated with PBS, free DOX, blank NP, or DOX-loaded NP (DOX-NP) at an equivalent drug dose of 0.2 mg/kg (dose 1) or 1 mg/kg (dose 2). At the end point, the extension of the osteolytic area was determined by X-ray analysis; sections of the tibiae were histologically examined to evaluate tumor area and the number of osteoclasts on the bone surface. (A) tumor area (mean ± SD); (B) osteolytic areas quantified on X-ray images of mice treated with dose 1 (mean ± SE); (C) number of osteoclasts (OcN/BS) found on bone surface in TRAP stained sections of the tibiae (mean ± SD). *p < 0.05, **p < 0.005 (adapted from reference [38]).