Table 6.
Comparison of superantigen detection by PCR and ELISA in S. aureus endocarditis isolates.
| Isolate | Gene detected by PCR | Superantigen detected by ELISA1
|
|
|---|---|---|---|
| Planktonic growth (OD405) | Biofilm growth (OD405) | ||
| 4297 | sea, see | SEA (0.747), SEE (0.189) | SEA (1.397), SEE (0.268) |
| 4319 | sea | SEA (0.564) | SEA (1.590) |
| 4578 | sea | SEA (0.517) | SEA (1.437) |
| 4420 | seb | SEB (2.281) | SEB (2.597) |
| 4587 | seb | SEB (0.334) | SEB (2.364) |
| 5494 | seb | SEB (0.334) | SEB (2.220) |
| 4320 | sec | SEC (0.716) | SEC (4.000) |
| 4576 | sed | SED (0.421) | SED (0.496) |
| 6517 | sed | Negative | SED (1.636) |
| 6609 | sed | SED (0.316) | SED (3.308) |
| 6613 | sed | Negative | SED (2.887) |
| 6728 | sed | SED (1.512) | SED (3.504) |
| 6982 | sed | SED (1.073) | SED (3.803) |
| 4288 | None | Negative | Negative |
| 4295 | None | Negative | Negative |
| 4329 | None | Negative | Negative |
| 4331 | None | Negative | Negative |
| 4334 | None | Negative | Negative |
| 4335 | None | Negative | Negative |
| 4339 | None | Negative | Negative |
| 4341 | None | Negative | Negative |
| 4372 | None | Negative | Negative |
| 4408 | None | Negative | Negative |
| 4410 | None | Negative | Negative |
| 4577 | None | Negative | Negative |
| 5417 | None | Negative | Negative |
| 5525 | None | Negative | Negative |
| 6512 | None | Negative | Negative |
| 6523 | None | Negative | Negative |
| 6528 | None | Negative | Negative |
1
OD405 <0.2 considered negative