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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Cancer Res. 2014 Feb 13;74(7):2082–2093. doi: 10.1158/0008-5472.CAN-12-0452

Figure 1. RhoE expression is down-regulated in SCC cell lines and tumors in parallel with Notch1 levels.

Figure 1

A, Primary HKC were analyzed in parallel with SCC cell lines (SCCO28, Cal27 and FaDu) by real time RT-PCR for Notch1 and RhoE mRNA levels using 36B4 as internal control. B, The same set of cells as in A, were analyzed for protein levels of Notch1 and RhoE using β-actin as a loading control. In addition protein levels of the cleaved part of Notch1 were detected with Val1744 specific antibody (lower panel). C, Total RNA from 23 surgically excised oral SCC subjected to laser dissection microscopy (as in (5) were analyzed along with normal epithelium for RhoE mRNA expression by real time RT-PCR using 36B4 as control. The results are expressed as fold changes of RhoE expression in the tumors versus normal epithelium. Error bars represent Standard Deviations, calculated based on triplicate values.