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. Author manuscript; available in PMC: 2015 Apr 25.
Published in final edited form as: Circ Res. 2014 Mar 18;114(9):1398–1409. doi: 10.1161/CIRCRESAHA.114.303870

Figure 4. PKA-dependent phosphorylation of PKD.

Figure 4

(A) In vitro phosphorylation of purified PKD with the catalytic subunit of PKA. Phosphorylation is measured with 2 different PKA consensus motif antibodies (RRXS*/T* top and RXXS*/T* bottom). (B) ISO- and Forsk-induced phosphorylation of immunoprecipitated GFP-PKD1 measured with a PKA consensus motif antibody (RRXS*/T*). Blots are probed with GFP for loading control. (C) Control immunoprecipitations (beads only and control IgG) demonstrate specificity of the PKD pull-down. (D) Potential PKA phosphorylation sites on mouse PKD1 sequence.