Figure 3. Investigating the mechanism of inhibition of lectin pathway activation via the MBL complex.

To determine if MASP-1 (A) or MASP-2 (B) remain associated with MBL in the presence of SMIPP-S D1 or I1 increasing concentrations of immobilised SMIPP-S protein or BSA as a negative control were incubated with MBL complexed with MASPs. MASP-1 and MASP-2 were then detected with specific antibody. The means of three independent experiments performed in duplicate +/− standard deviation (SD) are shown. Statistical significance of observed differences was calculated by two-way ANOVA and Bonferroni post test. *, p<0.05, **, p<0.01, ***, p<0.001, ns, p>0.05.