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. 2014 May 22;8(5):e2872. doi: 10.1371/journal.pntd.0002872

Figure 5. SMIPP-S mutants have lost inhibitory function in the lectin pathway.

Figure 5

To determine if D1 mutants (A) and I1 mutants (B) had lost inhibitory function in the lectin pathway deposition assays were performed. Microtitre plates coated with 100 µg/ml mannan were incubated with 2% NHS pre-incubated with 5 µg/ml D1 (positive control) or D1 mutant (A), 25 µg/ml I1 (positive control) or I1 mutant, or BSA as a negative control. Complement activation was assessed by detection of deposited MBL with specific antibody. The means of three independent experiments performed in duplicate +/− standard deviation (SD) are shown. Statistical significance of observed differences was calculated by two-way ANOVA and Bonferroni post test. *, p<0.05, **, p<0.01, ***, p<0.001, ns, p>0.05.