Table 2. Information of used primers.

Primer name	Primer sequences	Primer position (on genomic sequence of E. granulosus (G1) allele 1 (KF528663)	Product size (bp)
1. Primers to amplify p29 gDNA
Echi gP29F Forward primer (5′-3′)	TAAGCTGTGGGAACTAGTT	P −47 upstream of the start-ATG	1302
Echi gP29R Reverse primer (5′-3′)	TATGTGAACAAGCTAACAGG	P+55 downstream of the stop-TAG
2. Primers to amplify p29 cDNA
Echi cP29F Forward primer (5′-3′)	ATGTCCGGATTTGACGTTACTAAG	Exon 1: P1–P24	732
Echi cP29R Reverse primer (5′-3′)	CTACTCGCCCAGCATCATCATACT	Exon 7: P1201–P1181
3. Primers used for sequencing
Echi P29E5R Reverse primer (5′-3′)	CTGTTCCGCAGTCTTAG	Exon 5: P864–P848
T7 Forward	TAATACGACTCACTATAGGG
SP6 Reverse	ATTTAGGTGACACTATAG
4. MAS-PCR primers
EgG1P29A1F Forward primer (5′-3′)	CAGCAAGATCATCACC *	Exon2: P179–P194	195
EgG1P29A1R Reverse primer (5′-3′)	AAGACAACACCATATCTTAG *	Intron 2: P374–P355
EgG1P29A2F Forward primer (5′-3′)	ACCCTATTCATTTTTGCT *	Intron 3: P525–P542	258
EgG1P29A2R Reverse primer (5′-3′)	TTTCTAAGTGGGATAAAGG *	Intron 4: P783–P765
5. Primers to amplify p29 cDNA (for protein expression)
CACC-p29-Forward (5′-3′)	CACCATGTCCGGATTTGACGTTACTAAG
p29-Reverse (5′-3′)	CTACTCGCCCAGCATCATCATACT

*Allele specific 3′-base in each primer.