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. 2014 May 22;9(5):e97599. doi: 10.1371/journal.pone.0097599

Figure 9. Nuclear translocation of nuclear factor kappa B (p65) after IR or IR+FA by western blot and IHC.

Figure 9

Lane 1: control group of mice, lane 2: FA treated mice, lane 3, 4 and 5: Mice exposed to 10 Gy γ-radiation and sacrificed after 6 hours, 24 hours, and 48 hours of radiation exposure (IR6, IR24 & IR48). Lane 6, 7 and 8: mice pretreated with FA plus irradiated (FA+IR6, FA+IR24 & FA+IR48). Error bars were SEM for n = 3. p<0.05 was considered significant. Statistical comparison: * control vs. IR, #IR vs. FA+IR [Fig 9(a)]. NF-kB (p65) activation and entry into the nucleus of mice liver sections were determined by immunohistochemistry [Fig 9(b)]. Control: without any treatment, IR6, IR24 and IR48: mice irradiated with 10 Gy γ -radiation, FA: mice treated with FA (50 mg/kg bodyweight) for 5 days and IR6+FA, IR24+FA and IR48+FA: FA treated plus irradiated. NF- κB positive nuclei were estimated from the similar selected region.