(A) Multi-dimensional scaling plot showing relatedness between transcript expression profiles of Brugia malayi life-cycle stages, including previously reported mammalian stages [30]. Read counts resulting from immature and mature microfilariae (IM and MM), mosquito stages between day 1 and 4 (D1–4), and between day 4 and 8 (D4–8) were combined to generate representative expression profiles enriched for microfilariae (MF), L1, and L2, respectively. AM and AF, adult male and female; EE, eggs/embryos. The resulting data set (indicated in black) were used to examine (B) B. malayi genes differentially transcribed during the mosquito stages relative to the mammalian stages (p<0.01).