Table 1. Mutation frequencies in MLV and MMTV proviruses in splenic DNA of infected A3A and A3G mice.
Total sequenced | unique clones | # mice | # w/G to A | # w/o G to A | # G to A mutations | # other mutations | G to A kb | Other kb | GG | GA | GC | GT | |
A3Ghigh M-MLV | 56 | 42 | 4 | 32 | 24 | 158 | 34 | 5.14 | 1.11 | 149 | 7 | 1 | 1 |
A3Glow M-MLV | 47 | 28 | 4 | 23 | 24 | 35 | 48 | 1.33 | 1.82 | 33 | 2 | 0 | 0 |
A3Ahigh M-MLV | 49 | 9 | 4 | 0 | 49 | 0 | 7 | 0.00 | 0.26 | 0 | 0 | 0 | 0 |
A3Alow M-MLV | 47 | 17 | 4 | 4 | 43 | 4 | 38 | 0.16 | 1.47 | 0 | 1 | 1 | 2 |
KO M-MLV | 54 | 24 | 4 | 2 | 52 | 2 | 34 | 0.07 | 1.15 | 1 | 0 | 0 | 1 |
A3Ghigh MMTV | 57 | 40 | 4 | 29 | 28 | 68 | 59 | 1.77 | 1.54 | 58 | 8 | 1 | 1 |
A3Glow MMTV | 52 | 31 | 4 | 19 | 33 | 27 | 58 | 0.77 | 1.66 | 14 | 4 | 8 | 1 |
A3Ahigh MMTV | 52 | 29 | 4 | 6 | 46 | 12 | 62 | 0.34 | 1.77 | 1 | 10 | 1 | 0 |
A3Alow MMTV | 41 | 20 | 3 | 4 | 37 | 4 | 46 | 0.14 | 1.67 | 2 | 1 | 1 | 0 |
KO MMTV | 40 | 20 | 3 | 3 | 37 | 3 | 64 | 0.10 | 2.16 | 1 | 2 | 0 | 0 |
A3Ghigh F-MLV | 41 | 25 | 3 | 27 | 14 | 116 | 43 | 4.83 | 1.79 | 113 | 2 | 1 | 0 |
A3Ghigh F-MLV-vif | 53 | 23 | 4 | 6 | 47 | 12 | 71 | 0.39 | 2.29 | 11 | 0 | 0 | 1 |
A3Glow F-MLV | 40 | 18 | 3 | 18 | 22 | 27 | 19 | 1.15 | 0.81 | 21 | 1 | 2 | 3 |
A3Glow F-MLV-vif | 41 | 16 | 3 | 1 | 40 | 2 | 28 | 0.08 | 1.16 | 1 | 1 | 0 | 0 |
KO F-MLV | 42 | 11 | 3 | 3 | 39 | 7 | 37 | 0.28 | 1.50 | 5 | 1 | 1 | 0 |
KO F-MLV-vif | 42 | 42 | 3 | 1 | 41 | 1 | 67 | 0.04 | 2.72 | 1 | 0 | 0 | 0 |
Analysis was performed on the cloned DNA represented in Figure 4 (3–4 mice/group; 10–15 sequences/mouse). Unique clones were distinguished by their mutation pattern. M-MLV has a total of 47 GG and 33 GA motifs in the 549bp target sequence, MMTV has 34 GGs and 56 GAs in 673bp and F-MLV has 58 GG and 29 GA motifs in 586bp.