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. 2014 May 22;10(5):e1004151. doi: 10.1371/journal.ppat.1004151

Figure 5. BCA2 promotes the lysosomal degradation of HIV-1 and SIV Gag.

Figure 5

To determine if BCA2 promotes the proteasomal or lysosomal degradation of retroviral Gag proteins, virus release assays for HIV-1 and SIV were performed in the presence of proteasomal inhibitors (A-B) and lysosomal inhibitors (C-D). Virus release was measured by HIV-1 p24 or SIV p27 antigen-capture ELISA and expressed as the percentage of maximal virus release in the absence of HA-BCA2. The cell lysates (WCL) and virions derived from these transfections were analyzed by western blot. Clasto: clasto-Lactacystin β-lactone. Chlor: chloroquine. Error bars represent standard deviation of independent experiments.