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. 2014 May 22;10(5):e1004151. doi: 10.1371/journal.ppat.1004151

Figure 6. The targeted depletion of endogenous BCA2 results in increased virus release and replication.

Figure 6

Endogenous BCA2 was depleted by shRNA from 293T cells (A) and HOS cells (B) by transient transfection. Cells were subsequently transfected with HIV-1 NL4-3 or SIVmac239 proviral DNA, and virus release was measured 48 hours later. Depletion of endogenous BCA2 was also achieved in CD4+ T cells by transduction. Next, virus replication was assessed for HIV-1 in Jurkat cells (C) and for SIV in 221 T cells (D) by HIV-1 p24 and SIV p27 antigen-capture ELISA, respectively, at selected time points. The depletion of endogenous BCA2 was confirmed by western blot for each of these cell lines, by comparing the levels of endogenous BCA2 in the whole cell lysate (WCL) to those of β-actin. Error bars represent standard deviation of independent experiments.