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. Author manuscript; available in PMC: 2015 Feb 3.
Published in final edited form as: Mol Pharm. 2014 Jan 6;11(2):545–559. doi: 10.1021/mp4005035

Figure 7.

Figure 7

Pyridinium GS promotes endolysosomal escape of the GS-containing lipoplexes: confocal images of NCI-H23 cells (excitation 488 nm) expressing the endolysosomal protein GFP-Rab 7 transfected with select lipoplex formulations indicated in each case; Rab-7 protein is specific for endolysosomes [57,58] as its specific distribution can be observed in control NCI-H23 cells that did not receive lipoplexes (left panels). Lipoplexes that do not contain GS do not change significantly Rab-7 intracellular distribution (central panels) while introduction of GS in the structure of lipoplexes promotes endolysosomal membrane rupture and consequently a diffuse distribution of Rab-7 in the cytoplasm, irrespective of the co-lipid used and its molar ratio in lipoplex formulations (right panels).