Figure 5.

Changes in glutamate transporter expression during brain development. GLAST, GLT-1, EAAC1, and EAAT4 protein expression were analyzed in rat forebrain and cerebellum tissue from E16 to adult age (2 months). The figure represents four identical immunoblots (of the same samples) probed with antibodies to the various glutamate transporter subtypes as indicated. Note that the EAAC1 concentration in the forebrain is at its highest at P14. Similar blots were made from two other sets of animals with the same result (data not shown). Abbreviation: A, Adult. Each lane contained 20 μg of protein, and the blots were developed with antibody: anti-A522 (Ab#314; 0.2 μg/ml) for GLAST and anti-B12 (Ab#291; 0.2 μg/ml) for GLT-1; anti-C491 (Ab#237; 1 μg/ml) for EAAC1 and anti-D537 (Ab#181; 1 μg/ml) for EAAT4.