Table 1.

Isolation of EAAC1 protein from rat kidney

Detergent	Rat age	Antibody ID	Amount of pure EAAC1 protein obtained (μg)	WGA	DEAE	Sample ID
Cholate	220–240 g	Ab#237	88	No	Yes	1374
SDS	9 weeks	Ab#555	12	Yes	No	1357
SDS	9 weeks	Ab#555	20	Yes	No	1358

Rat kidneys were solubilized in cholate or SDS and EAAC1 isolated using immobilized anti-C491 antibodies (either Ab#237 or Ab#555). The bound EAAC1 protein was eluted at low pH, neutralized, and further purified by chromatography on either a wheat germ agglutinin lectin (WGA) column or a DEAE column as indicated. The age is given in weight (grams) or in weeks.