Table 1.
Primers and probes for genotyping
| Polymorphism | Position | WT>variant | Primer or Probe | |
|---|---|---|---|---|
| UGT1A1*6 | 211 | G>A | C 559715 20 | |
| UGT1A1*27 | 686 | C>A | C 2307598 20 | |
| UGT1A1*28 | TATA box | TA6>TA7 |
F-FAM R |
5′-gtgacacagtcaaacattaacttgt-3′ 5′-gcctttgctcctgccagaggtt-3′ |
| UGT1A1*60 | −3279 | T>G | C 1432134 10 | |
| UGT1A1*93 | −3156 | G>A |
F R FAM VIC |
5′-acttaacattgcagcacagg-3′ 5′-atgggcaaaagccttgaact-3′ 5′-cctgtccaagctca-3′ 5’-cacctgtctaagctca-3’ |
| UGT1A7*2 (N129K) UGT1A7*3 (W208R) |
387 622 |
T>G T>C |
F R |
5′-tacactctggaggatcagga-3′ 5′-tattgggcatcacgggtttg-3′ |
| UGT1A7*12 | −57 | T>G | C 287265 10 | |
| UGT1A9* 22 | −188 | T9>T10 | F | 5′-acttaacattgcagcacagg-3′ |
| R | 5′-atgggcaaaagccttgaact-3′ |
Nucleotide positions are relative to the transcription start site
Transposition of T to G at position 387 (or N129K) is termed UGT1A7*2. Transposition of T to C at position 622 is termed W208R, and co-occurrence of N129K and W208R is termed UGT1A7*3
TA6 6 TA repeats, TA7 7 TA repeats, F-FAM forward primer-labeled reporter 1 probe, F forward primer, R reverse primer, FAM reporter 1 probe, VIC reporter 2 probe, WT wild type