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. 2014 May 23;4:5043. doi: 10.1038/srep05043

Figure 1. TALEN design for inducing DSBs in the endogenous IL2RG gene in Jurkat cells.

Figure 1

(a) Schematic of the TALEN target site at the IL2RG start codon in Jurkat cells. IL2RG contains eight exons. The binding sites for the TALEN pair used in this study are shown in capital letters, the spacer sequence is indicated in small letters, the start codon (ATG) is in bold, and the TALEN target designed by ZiFiT in the spacer sequence is underlined. (b) Functional evaluation of engineered TALENs using the SSA assay. HEK293T cells were transfected with the SSA reporter vector, pRL-TK reference vector, and TALEN expression vectors. After 48 h, a luciferase assay was performed. Data are shown as mean ± SD (n = 3).