Table 2.
Specific enzyme activity of GDH in C. werkmanii DSM17579 (WT) and C. werkmanii DSM17579 ∆ dhaD (KO)
| |
Aerobic condition |
Anaerobic condition |
||
|---|---|---|---|---|
| Glycerol | Glycerol + glucose | Glycerol | Glycerol + glucose | |
| WT (U/mg) |
1.406 ± 0.283 |
0.005 ± 0.002 |
3.770 ± 0.259 |
1.293 ± 0.214 |
| KO (U/mg) | 0.007 ± 0.003 | BDL | No growth | 0.022 ± 0.005 |
Both strains were grown under (an)aerobic conditions in media with 163 mM glycerol only or 54 mM glucose and 163 mM glycerol (0.33 molar ratio glucose/glycerol) as carbon sources; BDL = below the detection limit.