Table 4.

Effect of the glucose/glycerol ratio on pH and yield; and carbon balance for glycerol utilization

Molar ratio glucose/glycerol	Yield (mol PDO/mol glycerol)	pH	Carbon balance glycerol (%)
1	0.90 ± 0.04	5.68 ± 0.05	95.51 ± 0.13
0.33	0.68 ± 0.05	6.07 ± 0.06	109.09 ± 1.26
0.2	0.43 ± 0.12	5.73 ± 0.24	99.80 ± 1.42
0.1	0.37 ± 0.07	5.94 ± 0.02	99.03 ± 0.35

C. werkmanii DSM17579 ∆dhaD was grown in medium with several molar ratios glucose/glycerol under anaerobic conditions on flask scale. The errors represent the standard deviation calculated from two independent experiments.