Skip to main content
. 2014 Apr 9;289(21):14458–14469. doi: 10.1074/jbc.M114.548156

FIGURE 4.

FIGURE 4.

AtPARK13 is a protease that prefers misfolded proteins. A, Coomassie-stained SDS-polyacrylamide gel showing purified AtPARK13 and the purified fraction from the pET28 empty vector control. B, protease activity assays showing that AtPARK13 has significant protease activity toward casein, which is not affected by increased temperature. C, protease activity assays showing that AtPARK13 preferentially degrades misfolded casein. D, Lineweaver-Burk plot showing the Vmax and Km for AtPARK13 in response to misfolded casein. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Error bars, S.E., n = 3.