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. 2014 Apr 9;289(21):14520–14533. doi: 10.1074/jbc.M113.532887

FIGURE 9.

FIGURE 9.

The CSC self-renewal capacity, cell migration, and the expression of EpCAM and Snail in the triple-marker-negative cells and the CSLC triple-marker-positive cells derived from L3.6pl cells under different conditions. Sphere formation assay and wound healing assays were performed to evaluate the CSC self-renewal capacity (A) and cell migration (B), respectively, in the triple-marker-negative cells and the CSLC triple-marker-positive cells derived from L3.6pl cells under different conditions. The quantitative RT-PCR assay (C) and confocal image microscopy (D) were performed to examine the expression of EpCAM and Snail in these cells. The transfection of FoxQ1 siRNA was performed in the CSLC triple-marker-positive cells derived from L3.6pl cells, as described under “Materials and Methods.” Error bars, S.D. *, p ≤ 0.05 (n = 3).