TABLE 4.
Characteristics of mouse xenograft tumors derived from parental and CSLC triple-marker-positive cells derived from MiaPaCa-2 cells
4-week-old SCID mice were transplanted with MiaPaCa-2 cells under different conditions. One mouse had spontaneous death in this group (MiaPaCa-2, plus FoxQ1 siRNA). After 34 days of inoculation, the animals were sacrificed, and the tumor was analyzed for histology, Western blot analysis, and quantitative RT-PCR analysis.
| Cell numbers of inoculation per animal | Tumor incidence (5 animals/group) | Tumor weight (mean ± S.D.) | p value | |
|---|---|---|---|---|
| mg | ||||
| Parental MiaPaCa-2, siRNA control | 2 × 106 | 4/5 | 333.5 ± 163.6 | <0.05a |
| Parental MiaPaCa-2, FoxQ1 siRNA | 2 × 106 | 0/5 | 0.0 ± 0.0 | |
| CSLC, siRNA control | 2 × 104 | 4/5 | 874.8 ± 504.4 | |
| CSLC, FoxQ1 siRNA | 2 × 104 | 1/5 | 126 ± 0.0 | <0.05b |
a,b p values less than 0.05 of the CSLC control group, compared with the MiaPaCa-2 control group and CSLC FoxQ1 siRNA group, respectively.