TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| S. oneidensis strains | ||
| MR-1 | Wild type | Laboratory stock |
| HG3237 | flaB deletion mutant derived from MR-1; ΔflaB | Ref. 13 |
| HG3238 | flaA deletion mutant derived from MR-1; ΔflaB | Ref. 13 |
| HG3961 | rpoN deletion mutant derived from MR-1; ΔrpoN | Ref. 32 |
| FFM | flaA and flaB deletion mutant derived from MR-1; ΔflaAΔflaB | Ref. 13 |
| Other bacterial strains | ||
| WM3064 | E. coli donor strain for conjugation; ΔdapA | W. Metcalf (University of Illinois at Urbana—Champaign) |
| JB580v | Y. enterocolitica wild type | Ref. 36 |
| GY4757 | Y. enterocolitica JB580v ΔyplAB, pYV8081− | Ref. 36 |
| Plasmids | ||
| pHG101 | Promoterless broad-host Kmr vector | Ref. 13 |
| pHG102 | pHG101 containing the S. oneidensis arcA promoter | Ref. 13 |
| pBBR-Cre | Helper vector for removing antibiotic marker | Ref. 32 |
| pHGE-Ptac-PetAGFP | Donor vector for the gfp gene | Ref. 34 |
| pHGEI01 | pHGC01 containing the full-length E. coli lacZ gene | Ref. 33 |
| pHGEI02 | pHGC01 containing the full-length gfp gene | Ref. 33 |
| pHG101-flaB | pHG101 containing the S. oneidensis flaB gene and its native promoter | Ref. 13 |
| pHG101-flaA | pHG101 containing the S. oneidensis flaA gene and its native promoter | This study |
| pHG101-PflaB-flaA | pHG101 containing the S. oneidensis flaA gene and the flaB promoter | This study |
| pHG102-flaA | pHG102 containing the S. oneidensis flaA gene | This study |
| pHGEI01-flaA | pHGEI01 containing the S. oneidensis flaA gene and its native promoter | This study |
| pHGEI01-flaB | pHGEI01 containing the S. oneidensis flaB gene and its native promoter | This study |
| pCSP50 | the E. coli--Y. enterocolitica shuttle vector | Ref. 36 |
| pCSP50-flaA-yplA | pCSP50 containing the fused flaA-yplA | This study |
| pCSP50-flaB-yplA | pCSP50 containing the fused flaB-yplA | This study |