TABLE 1.
Enzymological parameters of the wild-type and mutant β-Gals, and inhibitory constant values of PC compounds
The enzyme activity of β-Gal was fluorometrically determined with 4-methylumbelliferyll-β-d-galactopyranoside as a substrate.
| Specific activity | Vmax | Ki |
Ki |
NOEV | 6S-NBI-DGJ | 6S-NBI-GJ | NBT-DGJ | ||
|---|---|---|---|---|---|---|---|---|---|
| Galactose | DGJ | ||||||||
| μmol/min/mg | μm/min | mm | mm | μm | |||||
| WT | 15.6 | 1.78 | 0.49 | 4.14 | 61.8 | 1.1 | 68.3 | 253.1 | 372.7 |
| R201C | 13.1 | 1.74 | 0.44 | 5.61 | 49.0 | 0.94 | 53.3 | 320.4 | 388.3 |
| I51T | 16.3 | 1.82 | 0.55 | 4.91 | 44.7 | 1.2 | 68.8 | 319.6 | 329.0 |