FIGURE 4.

Enhanced auotcrine IL6 contributes to differentiation defects of dendritic cells with VentX knockdown. Human primary monocytes were transfected with VentX MO or control MO as indicated. Twenty-four hours post-transfection, cytokines GM-CSF plus IL4 were added into culture medium together with neutralizing IL6 antibody or control immunoglobulin (IgG). Cells were harvested 5 days after cytokine addition and analyzed for the expression of CD14 and CD1a by flow cytometry (left panel). The mean ± S.D. of four independent experiments is shown in the right panel.